from tkinter import *
import random

def use():
    if buttons[guzik] == min(buttons.values()):
        guzik.config(state = 'disabled', bg='green')
        #buttons.pop(guzik) #  error
    else:
        guzik.config(state = 'disabled', bg='red')
    
num = [x for x in range(1000)]
buttons = {}

root = Tk()

for i in range(25):    
    guzik = Button(root, text = random.sample(num,1), height = 1, width = 7)
    guzik.grid(row = int(i/5), column = i%5)
    buttons[guzik] = int(guzik.cget('text'))
    
print(buttons)

root.mainloop()