gene flow between population holds a species together genetically reproductive isolation when bio barriers prevent two species fron interbreeding. pre zygotic barrier habit iso temproal iso mechanical iso gametic iso postzygotic barrier, might not be viable reduced hybrid viability reduced hybrid fertility, liger sterile hybrid breakdown, genetic problems in F2 like deformities or sterilization reinforcement should be stronger for sympatric than allopatric populations fusion: weakened reproductive barrier